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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been noticed in several tumors, which ends up in chromosome instability, and thus may possibly contribute to tumorigenesis. This protein was found to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant position in promoting mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two extra splice variants were being documented but in depth nucleotide sequence information will not be but out there.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized through the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible results are...
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This information outlines even further doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
Antibodies bound to the blocking peptide not bind into the epitope over the concentrate on protein. By evaluating the staining through the blocked antibody as opposed to the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs into the highly conserved cyclin household, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which add on the temporal coordination of every mitotic occasion. Cyclin E sorts a complex with and functions being a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary which is degraded as cells progress by means of S stage.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation website by the two antibodies...
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